This procedure can be modified for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Spectrum chloroquine Chloroquine and hearing loss Plaquenil causes lowcalcium levels Hydroxychloroquine 200 mg acetaminophen 400 mg Transfection studies with PEI 2 kDa, PLL 3.4 kDa and PEI 2 kDa–melittin were performed both in the presence and absence of chloroquine. After 4 h, the mixture containing complexes was discarded and 500 µl of fresh medium containing 10% FCS was added to each well. Cells were cultured for 6–48 h prior to analysis of reporter gene expression. This protocol is a modified version of a published method4, in which calcium phosphate–based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells. For CaPO3, I use chloroquine, which inhibits DNA degradation by lysosomes by raising their pH - but this is toxic to the cell, so media must be changed after 6-8 hr. PEI also prevents DNA. Test a variety of brands and lots of FBS to find one suitable with your protocols. Last Upload: August 26, 2016 *Pro-Tip* Different brands and lots of FBS can promote or inhibit transfection. Chloroquine pei transfection Chloroquine diphosphate salt - Sigma-Aldrich, Calcium phosphate–mediated transfection of eukaryotic cells. Can hydroxychloroquine cause a false positive for pregnancyPlaquenil for lupus nephritisHydroxychloroquine to treat hair lossEye exam plaquenil This protocol can be used to produce lentivirus from a lentiviral vector transfected into Lenti-X 293T cells using a polyethyenimine PEI transfection protocol. This procedure can be modified for alternative packaging cell lines or transfection reagents. Addgene Lentivirus Production Protocol. How do you do transfection for lentivirus production?. Transfection efficiency and toxicity of polyethylenimine in.. Optimized PEI-based transfection reagent for scalable virus production. With its unique property to efficiently condense several plasmids of different sizes, PEIpro ® transfection reagent is ideal for optimal co-transfection of several plasmids containing the gene of interest and necessary viral components to produce full recombinant virions. Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development. Our results are supported by the view that chloroquine does not improve the transfection efficiency of PEI. Further, when transfection was carried out in the presence of bafilomycin A1, an ATPase pump inhibitor, a marked reduction in GFP expression was observed in both PEI and PEI-alginate nanocomposites.