Knowing a drug’s potential for partitioning is critical in situations where the test drug has a high Log P (lipophilic). In this situation a significant portion of the drug may be taken up and held by red blood cells (RBCs). Drug of choice for chloroquine resistant malaria in pregnancy Plaquenil & hrt Das psychosis induced by hydroxychloroquine Can erythromycin interact with plaquenil Readout Blood-to-Plasma ratio % Controls Verapamil. Assay Description Fresh blood EDTA-K2 is incubated in a humidified atmosphere of 5% CO 2 at 37 °C. 1 mL blood is removed into a 1.5 mL tube and centrifuged. A stock solution of the test compound and verapamil working solution with DMSO are spiked into 4 mL blood, respectively, and vortexed gently. The time to peak concentration and the maximum concentration varied in various tissues and were higher than for plasma and red blood cells. 3. 3. The tissue to plasma concentration ratio increased with time up to 144th hr for some tissues while this ratio was still increasing at the 168th hr for other tissues. 4. 4. Specifically, hematocrit is the percentage of blood volume that is cellular, with a minimum value of 0% and a maximum value of 100%. Normal hematocrit is approximately 48% for men and 38% for women. This means that men normally have 48% RBC and 52% plasma. The numbers for women are 38% RBC and 62% plasma. When the combined knowledge of protein binding and blood partitioning is understood investigators can make a better determination about whether they should collect whole blood, plasma, or serum for assaying pharmacokinetic behavior of the drug if animal or clinical ADME studies. For this reason, more informative data describing drug distribution in blood can be obtained by determining both the blood partitioning ratios and direct protein binding. Chloroquine blood to plasma ratio Dose Optimization of Chloroquine by Pharmacokinetic Modeling., Kinetics of the distribution and elimination of chloroquine. Plaquenil c 24-2 Chloroquine is rapidly and almost completely absorbed from the gastrointestinal tract, and only a small proportion of the administered dose is found in the stools. Approximately 55% of the drug inthe plasma is bound to nondiffusible plasma constituents. Excretion of Chloroquine is quite slow,but is increased by acidification of the urine. Chloroquine - FDA prescribing information, side effects.. Blood or Plasma? Which Should You Assay for Drug. - Certara. Dose Optimization of Chloroquine by Pharmacokinetic.. I have an assumption that plasma is a part of Blood and concentration in plasma should always be lessthan or equal to that in blood and hence, the ratio should be 1 or greater than 1. Thank you, Abhijith Back to the Top. Oct 01, 2018 Chloroquine is deposited in the tissues in considerable amounts. In animals, from 200 to 700 times the plasma concentration may be found in the liver,spleen, kidney, and lung; leukocytes also concentrate the drug. The brain and spinal cord, in contrast, contain only 10 to 30 times the amount present in plasma. It turns out that Vss, the total body clearance, Cl, equilibrium blood-plasma concentration ratio, r; and the physiological parameters of the body such as cardiac output, and blood and tissue.